What is Nagalase? by Dr. Nicol Giandomenico


Alpha-N-acetylgalactosaminidase, also known as nagalase, is an enzyme that is secreted by cancer cells to breakdown the extracellular matrix during tumor invasion. In addition, it is an intrinsic part of the envelope protein of certain viruses and  is also secreted by cells infected with virus. Nagalase deactivates the Gc protein which, once bound to vitamin D3, is converted to a major macrophage activating factor (MAF). By disabling MAF, nagalase prevents macrophage activation for phagocytosis and antigen presentation, leading to immune suppression.

Elevated nagalase is indicative of either tumor activity or an active viral load in the body. With suppressed macrophage activity, viruses can become highly active and create fatigue, low grade fevers, muscle aches, joint pains and continued immunosuppression. This is often an underlying cause of many chronic illnesses such as chronic fatigue syndrome, fibromyalgia and Lyme disease.

What is GcMAF and what are the treatment options with GcMAF?

GcMAF stands for Gc protein macrophage activating factor, and is the activated form of the Gc protein once it becomes bound with vitamin D. Nagalase can only destroy the Gc protein, not GcMAF, so the introduction of an external source of GcMAF via injection has been shown to be effective. GcMAF is available in two different forms as an injection.

GOleic is the GcMAF molecule combined with oleic acid, and is understood to be more effective in that form, or is available as injection as well as under the tongue. It can also be used in a nebulizer or made into suppositories.

GcMAF is also available in a yogurt. The Bravo Probiotic Yogurt contains 42 different strains of probiotic bacteria and yeast and is specially designed to contain lactoferrins, immunoglobulins and the GcMAF immunostimulating factor. This yogurt is designed to be lactose and casein free, but also comes as a suppository for those that are sensitive to cow’s milk.

A nagalase test every 8 weeks is recommended to monitor GcMAF activity. A decrease in nagalase indicates it is working, and a healthy level is below 0.65nmol/min/mg. Your doctor may test your nagalase levels if they suspect a high viral load contributing to your symptoms, and they will also test your Vit D levels to make sure they are optimal for the GcMAF protocol. Additionally, they may prescribe other immune enhancing factors such as amino acid supplementation, immunoglobulins such as in whey protein and colostrum that enhance the body’s uptake and production of GcMAF.